Molecular and Biochemical Detection of Streptococcus equi Involved in Strangles in Horses
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Abstract
Strangles is a life threatening highly contagious disease caused by Streptococcus equi (S. equi). The study was designed to quantify the equine infection caused by S. equi in horses. A total of 50 samples were aseptically collected from the abscess of sub-mandibular lymph nodes and analyzed for the presence of S. equi. For conventional diagnosis, morphological and biochemical tests were performed and for advanced molecular detection, PCR technique was adopted for final confirmation. The bacteria caused the haemolysis on blood agar plate, were spherical in shape, appeared as chains on gram staining and only fermented glucose and lactose. PCR results indicated that 9 out of 50 (18%) samples were contaminated with S. equi when spy1258 gene (407bp) was targeted for detection of the said pathogen. The study provides the primary insight of S. equi involved in strangles and will help to timely diagnose and treat the infected horses.
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